At 8.30 am, DRH Auditorium, 1st floor, Hospital premises...

• ELISA (Enzyme-linked immuno-sorbent assay) is a type of immunoassay, used for detection of Antibodies, Antigens , Proteins ,Peptides , Biomolecules

• Antigen  or antibody of interest is absorbed on to plastic surface (‘sorbent’)
• Antigen is recognised by specific antibody  or vice versa (‘immuno’)
• Antibody molecule is attached with an enzyme (‘enzyme-linked’)
• Substrate added at the end of the reaction
• Enzyme breaks it to coloured end products

• 1^st generation - Infected cell lysate
• 2^nd generation - Glycopeptides (recombinant antigen)
• 3^rd generation  - Synthetic peptides
• 4^th generation – Both Antigen and antibody detected

• Diagnosis of infections – almost any
• Detecting allergens in food and house dust
• Detection of autoantibodies  - collagen vascular diseases
• Measuring toxins in contaminated food
• Detecting illicit drugs like Cocaine , opiates or anabolic steroids

• Rapid diagnostic assays have a lot of drawbacks
• Need of the hour : SHIFT FROM RAPID DIAGNOSTIC TESTS TO ELISA
• Only drawback of ELISA is turnaround time