Microbiology

About the Department

Department of MICROBIOLOGY is involved in teaching, training, diagnostic and research activities. It investigates the samples from various patients for identification of infectious cause of the condition. The laboratory also caters to the private hospitals of Hubli and Dharwad.

The Microbiology laboratory is distributed in different sections like Bacteriology, Serology, Immunology, Mycobacteriology, Mycology, Virology, Parasitology and Molecular biology. Approximately 1lakh samples are evaluated every year at this laboratory. Most of the modern instruments and equipments like BacT/ALERT 3D automated blood culture system, several biosafety cabinets, ELISA system, Mini API, Mini VIDAS, a Leika fluorescence microscope, automated autoclave, electronic balances etc. are spread over a vast working area.

A state of art molecular biology laboratory with conventional as well as Real-Time PCR equipments is the pinnacle of this department.

In spite of having best of the equipments the management has offered the diagnostic services to the common people at a considerably low cost. The serology section performs the fourth generation ELISA test for diagnosis of AIDS. Immunology section has various tests for rapid diagnosis of many infections. A few significant isolations to clinch difficult diagnosis have been made by this department. Isolation and identification of Brucella species,Cladophialophora bantiana – a neurotropic fungus, Burkholderia pseudomallei, rareCandida species have been highly appreciated by experts across the country. Rapid diagnosis of malaria, dengue etc. is helping the clinicians to treat our patients effectively. We have established a PCR for diagnosis of Mycobacterium tuberculosisinfections and providing the test at a highly reasonable cost to the needy. Work on establishing the test for other infectious and non-infectious parameters is going on.

Staff of the department is involved in various research activities in the fields of their interest and have brought out publications in the form of books and major articles in the national and international journals. It was a proud privilege to conduct a state level CME on Diagnosis of Malaria on 12th June 2010. Department co-ordinates the infection control and antibiotic policy committees which are an integral part of this privileged institute. This department played an important role in bringing out antibiotic policy in the form of a printed booklet under the guidance of our Medical Director, Dr. Niranjan Kumar. We thus, joined the club of only a very few institutes having their own antibiotic policy in the country. 

 

Head of Department

Dr. Raghavendra D. Kulkarni, MD, is currently heading the department. Dr. Kulkarni has a vast teaching experience over 30 years. He has worked at institutes like Govt. Medical College, Miraj and Grant Medical College, Mumbai before joining this institute in 2005. He is a reputed researcher and has over 50 research publications to his credit; both in national and international journals. He is working as a principal and co-investigator on projects supported by the RGUHS, Bangalore and ICMR, New Delhi. He also writes in Marathi periodicals and magazines on scientific subjects. He has received advanced training in diagnostic molecular biology at the DRDO, Gwalior. Dr. Kulkarni has delivered guest lectures at a number of conferences and academic platforms at several places in the country.  He is the Associate Editor of Indian Journal of Research and Reports in Medical Sciences.
Dr. Kulkarni, is a guide for MD/PhD (Medical Microbiology) at RGUHS, Bangalore and Shivaji University, Kolhapur. He is the President of Indian Association of Medical Microbiologists for the year 2015-16. He also has held the post of Treasurer of Indian Association of Medical Microbiologists (National Body) from 2008 to 2011. Apart from teaching and research he is a passionate, amateur flutist. He is currently receiving training in Hindustani Classical style.
Dr. Kulkarni has undergone an advanced training in Molecular Biology at St. Giles Laboratory, Rockefeller University, New York from January 2013 to March 2013.   

 

Diagnostic Facilities

The Microbiology laboratory is distributed in different sections like Bacteriology, Serology, Immunology, Mycobacteriology, Mycology, Virology, Parasitology and Molecular biology. Approximately 70,000 samples are evaluated every year at this laboratory. Most of the modern instruments and equipments like BacT/ALERT 3D automated blood culture system, several biosafety cabinets, ELISA system, Mini API, Mini VIDAS, a Leika fluorescence microscope, automated autoclave, electronic balances etc. are spread over a vast working area. A state of art molecular biology laboratory with conventional as well as Real-Time PCR equipments is the pinnacle of this department. In spite of having best of the equipments the diagnostic services are offered to the common people at a considerably low cost. The serology section performs the fourth generation ELISA test for diagnosis of AIDS. Immunology section has various tests for rapid diagnosis of many infections. A few significant isolations to clinch difficult diagnosis have been made by this department. Isolation and identification of brucella species, Cladophialophora bantiana – a neurotropic fungus, Burkholderia pseudomallei, rare candida species have been highly appreciated by experts across the country. Rapid diagnosis of malaria, dengue etc. is helping the clinicians to treat patients effectively. PCR for diagnosis of Mycobacterium tuberculosis infections has been established and it is providing the test at a highly reasonable cost to the needy. Work on establishing the test for other infectious and non-infectious parameters is going on. The RNTCP laboratory of the institute which is located in the Department of Microbiology, has won the “Best RNTCP Center” in Karnataka prize. The ICTC centre for voluntary testing for HIV infection is also functioning in this department.

 

Teaching Program

Department is involved in teaching MBBS, MD, Nursing, Dental, Physiotherapy, DMLT and other paramedical courses. The teaching schedule is highly disciplined and punctual. Periodic assessment of the students, followed by counseling for better performance is given a lot of importance. The Department is well equipped with class rooms having audio-visual aids, demo rooms, and practical hall for effective teaching of medical students. The practical hall for undergraduate students is beautifully made with granite top working benches, excellent quality stains, slides and 60 top class microscopes for the use of students. An excellent museum is a prized possession of this department. Regular topic seminars, culture seminars, laboratory test seminars, sample seminars and journal clubs are conducted as a part of post graduate activity. A very well equipped PCR laboratory is available for training the post graduates in molecular techniques. Polyacrylamide gel electrophoresis, Western Blotting for protein profiles etc. is carried out here routinely.  The department insists on hands on training to the PGs in the conventional as well as modern techniques. In addition postgraduates are posted to various diagnostic divisions of the department. They are also deputed to other well known institutes for their training.

The faculty regularly gets updated by attending conferences, CMEs, seminars, training programs etc. The department has published 40 research papers in last five years.

 

Workshops and CMEs
  1. On 12th of June 2010 Department of Microbiology organized a state level CME on Diagnosis of Malaria. The speakers included Dr. Basavaraj V. Peerapur, Dr. Satish Amarnath, Dr. Mahantesh Nagmoti and Dr. Vijay Kumar. Delegates from Karnataka and Maharashtra attended the CME in large number. 
  2. Conducted “HANDS ON DNA PCR WORKSHOP” for Students of Biotechnology, Microbiology and Biochemistry from 19th February 2015 to 21st February 2015.

 

Publications
  1. Jain PA, Srinikethana G, Shubhada C, Pradeep K, Gopala M, Kulkarni R, Praveenchandra KR. Effective of five plants extracts on adhesion of candida albicans onto human buccal epithelial cells: an in-vitro study. Egyptian Pharmaceutical Journal. 2014; 13(2): 137-143.
  2. Appannanavar SB, Goyal K, Garg R, Ray P, Rathi M, Taneja N. Shigellemia in a post renal transplant patient: a case report and literature review. Journal of Infection in Developing Countries. 2014; 8(2): 237-239.
  3. Taneja N, Appannanavar SB, Kumar A, Varma G, Kumar Y, Mohan B, Sharma M. Serotype profile and molecular characterization of antimicrobial resistance in non-typhoidal salmonella isolated from gastroenteritis cases over nine years. Journal of Medical Microbiology. 2014 Jan; 63(Pt 1): 66-73.
  4. Kumar A, Biswal M, Dhaliwal N, Mahesh R, Appannanavar SB, Gautam V, Ray P, Gupta AK, Taneja N. Point prevalence surveys of healthcare-associated infections and use of indwelling devices and antimicrobials over 3 years in a tertiary care hospital in India. Journal of Hospital Infection. 2014; 86(4): 272-4.
  5. Gupta PK, Appannanavar SB, Kaur H, Gupta V, Mohan B, Taneja N. Hospital acquired urinary tract infection by multidrug-resistant Brevundimonas vesicularis. Indian Journal of Pathology and Microbiology. 2014; 57(3): 486-8.
  6. Biswal M, Rajpoot S, Dhaliwal N, Appananavar SB, Taneja N, Gupta AK. Evaluation of the short-term and long-term effect of a short series of hand hygiene campaigns on improving adherence in a tertiary care hospital in India. American Journal of Infection Control. 2014 Sep; 42(9): 1009-10.
  7. Sonth SB, Sathyanarayan, MS, Anuradha S. Kalabhavi, Surekha YA, Mariraj J, Krishna S. A study of dermatophytosis in medical college hospital, Bellary. International Journal of Current Research. 2013; 5(6): 1597-8.
  8. Kalabhavi AS. Chromoblastomycosis–review article. International Journal of Current Research. 2013; 5(7): 1691-5.
  9. Jain PA, Kulkarni RD, Swamy BM, Mugeraya G, Srinikethan G. Sharanabasu KA. A method for efficient extraction of cell wall proteins from candida albicans. International Journal of Current Research and Review. 2013; 5(16). 01-07.
  10. Ajantha GS, Patil SS, Chitharagi VB, Kulkarni RD. Rickettsiosis: a cause of acute febrile illness and value of Weil-Felix test. Indian Journal of Public Health. 2013; 57(3). 182-183.
  11. Patil SS, Mahale K, Shetty P, Nekar MS. Antibiotic susceptibility pattern of urinary isolates from a tertiary care hospital with special reference to gram negative bacteria. IOSR Journal of Dental and Medical Sciences. 2013; 12(1): 49-51.
  12. Pandey V, Shubhada C, Ajantha GS, Kulkarni RD. Comparison of enterococcal colonization of gut in hospitalized and non-hopitalized patients. Journal of Gastrointestinal Infections. 2013 June-July; 3(1):
  13. Ajantha GS, Shetty P, Kulkarni RD, Biradar U. PCR as a diagnostic tool for extra –pulmonary tuberculosis. Journal of Clinical and Diagnostic Research. 2013 Jun; 7(6): 1012–1015.
  14. Appannanavar SB, Biswal M, Rajkumari N, Mohan B, Taneja N. Evaluation of commercial boric acid containing vials for urine culture: low risk of contamination and cost effectiveness considerations. Indian Journal of Pathology and Microbiology. 2013; 56(3): 261-4.
  15. Biswal M, Singh NV, Kaur R, Sebastian T, Dolkar R, Appananavar SB, Singh G, Taneja N. Adherence to hand hygiene in high-risk units of a tertiary care hospital in India. American Journal of Infection Control. 2013 Nov; 41(11): 1114-5.
  16. Journal of Gastrointestinal Infections. 2013
  17. Sharma K, Sharma A, Appannanavar S, Modi M, Prabhakar S, Sharma M, Varma S. Mycobacterium avium meningitis in HIV patients in North India. AIDS Res Hum Retroviruses. 2013 June; 29(6): 849-50.
  18. Patil DP, lava R. Identification, characterization and antibiotic susceptibility of shigella species isolated from stool samples in children. International Journal of Biological and Medical Research. 2012; 3(2): 1640-3.
  19. Ajantha GS, Hegde V. Antibacterial Drug Resistance and its Impact on Dentistry. The New York State Dental Journal. 2012 Jun-Jul; 78(4): 38-41.
  20. Mahale K, Patil SS, Ravikumar, Nagabhushan, Mahale R. Prevalence of Cryptococcal Meningitis among Immuno-competent and Immuno-compromised Individuals in Bellary, South India: a prospective study. Journal of Clinical and Diagnostic Research. 2012 May; 6(3): 388–92.
  21. Jain PA, Mugeraya G, Srinikethan G., Swamy BM. Studies on adhesion activity of candida albicans to human buccal epithelial cells in relation to its growth phases. Journal of Bioscience and Technology. 2012; 3(1): 449-54.
  22. Shubhada C, Bale MurugeSwamy. Salivary expression of sclerotium rolfsiilectin–binding mucin-type glycoprotein in human oral cancer. Journal of Bioscience and Technology. 2012; 3(1): 455-61.
  23. Anehosur GV,  Kulkarni RD, Naik MG, Nadiger RK. Synthesis and determination of antimicrobial activity of visible light activated tio2  nanoparticles with polymethyl methacrylate denture base resin against staphylococcus aureus. Journal of Gerontology & Geriatric Research. 2012; 1(1): 103-11.
  24. Ajantha GS, Kulkarni RD, Kalabhavi AS, Shetty PC, Jain PA. A Laboratory Analysis of HIV–2 over a Period of Five years in a Tertiary Care Hospital and Review of Literature. International Journal of Biological and Medical Research. 2012; 3(1): 1263-6.
  25. 2012 May; 51(5): 576-8.
  26. Khurana S, Appannanavar SB, Bhatti HS, Verma S. Sparganosis of liver: a rare entity and review of literature. BMJ Case Reports. 2012 Dec;
  27. Appannanavar SB, Sharma K, Verma S, Sharma M. Seroprevalence of Brucellosis: a 10-year experience at a tertiary care center in north India. Indian Journal of Pathology and Microbiology. 2012; 55(2): 271-2.
  28. Mishra B, Varma N, Appannanavar SB, Malhotra P, Sharma M, Bhatnagar A, Ratho RK, Varma S. Viral markers in patients with hemophagocytosis: a prospective study in a tertiary care hospital. Indian Journal of Pathology and Microbiology. 2012; 55(2): 215-7.
  29. Patil SS, Mahale K, Krishna S, Mariraj J, Kalabhavi AS, Sathyanarayan MS. Evaluation of gram stain of uncentrifuged urine as a screening methods for diagnosis of urinary tract infections. Current Research in Medicine and Medical Sciences. 2011; 1(2): 19-21.
  30. Ajantha GS, Kulkarni RD, Upadhya AK, Kalabhavi AS, Patil SS, Shetty PC, Shubhada RM, Jain PA. Urinary tract infection in a tertiary care hospital with special reference to methicillin resistant S. aureus (MRSA). Indian Journal of Research and Reports in Medical Sciences. 2011; 1(1):22-6.
  31. Patil HV, Patil VC, Ramteerthkar MN, Kulkarni RD. Central venous catheter-related bloodstream infections in the intensive care unit. Indian Journal of Critical Care Medicine. 2011 Oct; 15(4): 213-23.
  32. Pradeeep K, Kidiyoor KH, Jain PA, Rao N, Rao PK. Chair side disinfection of gutta–percha points an in-vitro comparative study between five different agents at different concentrations. International Journal of Dental Update 2011; 1(1): 11-5.
  33. Kamalapur MG, Patil PB, Joshi SK, Satish M, Patil SH, Ajantha GS, Kulkarni RD. Role of magnetic resonance imaging in distinguishing fungal from nonfungal multiple brain abscesses. The Egyptian Journal of Radiology and Nuclear Medicine. 2011; 42(3): 397-4.
  34. Ajantha GS, Kulkarni RD. Polymerase Chain Reaction and its importance in Medicine. International Journal of Current Science. 2011; 1(4): 198-200.
  35. Anuradha SK, Surekha YA, Satyanarayan MS, Suresh S, Krishna S, Satish SP, Mariraj J. Ravikumar R. Japanese encephalitis virus–common cause of viral encephalitis in paediatric age group in Bellary, Karnataka, India. Journal of Clinical and Diagnostic Research 2011; 5(3): 480-2.
  36. Anuradha SK, Surekha YA, Satyanarayan MS, Suresh S, Satish P, Mariraj J, Krishna S, Ravikumar R. Epidemiological aspects of Japanese encephalitis in Bellary, Karnataka, India . International Journal of Biological & Medical Research. 2011; 2(3): 691-5.
  37. Nayak AG, Fernandes A, Kulkarni R, Ajantha GS, Lekha K, Nadiger R. Efficacy of antibacterial sealing gel and o-ring to prevent micro leakage at the implant abutment interface-an in vitro study. Journal of Oral Implantology. 2014 Feb; 40(1): 11-4.
  38. Anila K, Hallikeri K, Shubhada C, Naikmasur VG, Kulkarni RD. Comparative study of Candida in oral submucous fibrosis and healthy individuals. Rev Odonto Cienc. 2011; 26(1): 71-6.
  39. Jain PA, Kulkarni RD, Ajantha GS, Shubhada C. A Comparative Evaluation of Oral Candida Carriage in HIV-Infected individuals and HIV Seronegative Healthy individuals in North Karnataka. Journal of Bioscience and Technology. 2011; 2(2): 232-7.
  40. Ajantha GS, Kulkarni RD. Cladophialophorabantiana, the neurotropic fungus- a mini review. Journal of Clinical and Diagnostic Research. 2011; 5(6): 1301-6.
  41. Kulkarni RD, Patil SS, Ajantha GS, Upadhya AK, Kalabhavi AS, Shubhada RM, Shetty PC, Jain PA. Association of Platelet count and serological markers of dengue infection-importance of NS1 antigen. Indian Journal of Medical Microbiology. 2011; 29(4): 359-62.
  42. Ajantha GS. Antimicrobial drugs and the crisis of drug resistance. Health Sciences. 2011; 1(3): 19-20.
  43. Kulkarni RD, Ajantha GS, Upadhya AK, Patil SS, Kalabhavi AS, Shubhada RM, Shetty PC, Jain PA, Dutta S. Antibiotic susceptibility patterns of the three bacterial enteric pathogens isolated from a tertiary care hospital. Journal of Gastrointestinal Infections. 2011; 1(1): 43-5.
  44. Jain PA, Veerabhadrudu K, Kulkarni RD, Ajantha GS, Shubhada C, Amruthkishan U. Comparative study of adherence of oral Candida albicans isolates from HIV sero-positive individuals and HIV sero-negative individuals to human buccal epithelial cells. Indian Journal of Pathology Microbiology. 2010; 53: 513-7.
  45. Rao DD, Desai A, Kulkarni RD, Gopalkrishnan K, Rao CB. Comparison of maxillofacial space infection in diabetic and non-diabetic patients. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology. 2010; 110(4): e7-12.
  46. 2010; 131: 584-5.
  47. Kulkarni RD, Batra HV, Tuteja U, Shukla J, Patil SA, Kulkarni VA, Ramteerthakar MN, Kumbhar DV, Joshi PA, Madwanna VP, Jahagirdar VL. Investigation into an outbreak of acute febrile illness in Sangli district of Maharashtra, India. International Journal of Clinical Practice. 2010; 64: 95-6.
  48. Nagaraja N. Nagre, Vishwanath B. Chachadi, Sachin M. Eligar, C. Shubhada, RadhaPujari, Padma Shastry, Bale M. Swamy, Shashikala R. Inamdar. Purification and Characterization of a Mitogenic Lectin from Cephalosporium, a Pathogenic Fungus Causing Mycotic Keratitis. Biochemistry Research International. 2010; 6.
  49. Sreenivasan PK, DeVizio W, Prasad KV, Patil S, Chhabra KG, Rajesh G, Javali SB, Kulkarni RD.. Regional Differences Within the Dentition for Plaque, Gingivitis, and Anaerobic Bacteria. Journal of Clinical Dentistry. 2010; 21:13-9.
  50. Moolya NN, Thakur S, Ravindra S, Setty SB, Kulkarni R, Hallikeri K. Viability of bacteria in dental calculus-a microbiological study. Journal of Indian Society of Periodontology. 2010 Oct; 14(4): 222-6.
  51. Veena Kumari HB, Nagaraja D, Nagarathna S, Kulkarni GB, Praveen CS, Nadig S, Arakere G. A variant epidemic methicillin resistant Staphylococcus aureus-15 cavernous sinus thrombosis and meningitis: A rare occurrence with unusual presentation. Indian Journal of Medical Microbiology. 2010 Jul-Sep; 28(3): 255-7.
Dissertations/ Research
No. Topic Student Guide

1

Study of Neonatal Septicemia with Special Emphasis on Candidemia

Dr. Renuka Anegundi

Dr. RD Kulkarni

2

Study of Concordance Among Quantitative Buffy Coat (QBC), Antigen Detection and Peripheral Smear Examination for the Laboratory Diagnosis of Malaria

Dr. Vidyavathi  B Chitharagi

Dr. RD Kulkarni

3

A Comparative Evaluation of Polymerase Chain Reaction, Ziehl-Neelsen stain, auramine stain and culture in the diagnosis of Extrapulmonary Tuberculosis

Dr. Deepali Vagdalker

Dr. Ajantha GS

4

Isolation, speciation and antibiotic sensitivity pattern of Acinetobacter isolates from clinical samples

Dr. Sughosh Kulkarni

 

Dr. RD Kulkarni

5

Clinicomycological correlation of TineaCorporis

Dr. Akshatha T

Dr. Ajantha GS

6

Speciation  of Candida Isolates from Clinical Samples: A Comparison of two Carbohydrate Assimilation Methods for Efficacy, Speed and Accuracy with Special Reference to the Sensitivity to Commonly Used Antifungal Agents

Dr. Amith Joshi

Dr. RD Kulkarni

7

Identification of Biofilm Producing Staphylococcus Species from Clinical Samples by Three Different Phenotypic Methods and Comparison of their Antibiogram

Dr. DeepaRokhade

Dr. Ajantha GS

Research Grants received by faculty
  1. Dr. R. D. Kulkarni received RGUHS, project grant for his research proposal entitled “Comparative evaluation of oral colonization of candida in HIV positive and HIV Negative individuals  with special reference to fluconazole sensitivity” during 2009 - 10
  2. Dr. (Mrs.) Pavithra Jain received ICMR project grant for her research proposal entitled “Cell wall protein profiling of oral Candida albicans from HIV infected individuals using lectins”. The project work commenced from 1st of April 2011 (2010-11)
  3. Dr. (Mrs.) Pavithra Jain received RGUHS project grant for her research proposal entitled “Lectin typing ofCandida albicans from HIV infected individuals-an epidemiological study”. The project work commenced from 1st of June 2011 (2011 -12)
  4. Dr. R. D. Kulkarni, Co-investigator, Antimicrobial activity of TiO2 photo catalyst containing acrylic denture base material, ICMR Project. (2010-12)
  5. Dr. Shubhada C, Co – Principal Investigator, Diagnostic and therapeutic implications of miRNA mediated regulation of Prostate Cancer,  ICMR Adhoc Research Grant (2014-15)
  6. Dr. R. D. Kulkarni, Principal Investigator and Dr.Ajantha G.S, Co-Investigator.  Isolation, Identification and Differentiation of Different Pathotypes of DiarrheagenicE. coli from Clinical Samples and their Drug Resistance Profiling. Funded by DFRL, Mysore
Faculty
1
Dr. Raghavendra D. Kulkarni
Professor & HOD
2
Dr. Ajantha G. S.
Professor
3
Dr. Anuradha S Kalabhavi
Associate Professor
4
Dr. Praveen C. Shetty
Associate Professor
5
Dr. Manjunath A Hosamani
Assistant Professor
6
Dr. Suma B Appannavar
Assistant Professor
7
Dr. Deepa P. Patil
Assistant Professor
8
Dr. Shubadha C.
Assistant Professor (Msc,Phd)
9
Dr. Pavithra G. Jain
Assistant Professor (Msc,Phd)
10
Dr. Sughosh Kulkarni
Tutor
11
Dr. Deepa Rokhade
Tutor
12
Dr. Bhumika Chandra Lali
Tutor

 

 

 

 

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