Laboratory is well equipped to perform biomedical research in various fields like Molecular and Cellular biology, Immunology, stem cell biology, Biochemistry and Nanoparticle formulation and Characterization. The scope of the laboratory is to cater the research needs of graduate and postgraduate students and also interested faculty from all the departments of the Institution.
CENTRAL RESEARCH LABORATORY COMMITTEE
Chairman: Dr. Niranjankumar, Medical Director
Co-Ordinator: Dr. S. K. Joshi, Principal
Research Co-Ordinator: Dr. Praveenkumar Shetty Short CV
Members:
Dr. Satyashankar, Medical Superintendent
HOD, Pathology
HOD, Biochemistry
HOD, Pharmacology
HOD, Microbiology
NATIONAL & INTERNATIONAL COLLABORATIONS AT INVESTIGATOR LEVEL
UNT Health Science Center at Fort-Worth, Texas, USA
National Centre for Cell Sciences, Pune.
K. S. Hegde Institute of Medical Sciences, Nitte University, Mangalore.
Karnataka Cancer Therapy & Research Institute, Hubli
Karnataka University, Dharwad
Kuvempu University, Shivamogga, Karnataka
CURRENT FUNDING
ICMR Ad-hoc Research Project:
“Identification and characterization of Novel Biomarkers and Therapeutic targets for Triple Negative Breast Cancer”
Principal Investigator: Dr.Praveenkumar Shetty
Co-Investigators:
Dr. B. R. Patil, Karnataka cancer Therapy and Research institute, Hubli
Dr. Col. U. S. Dinesh, Dept. of Pathology, SDMCMSH
Dr. Pramod B. Gai, DNA Diagnostic laboratotry, Dharwad
DST-SERB: “Diagnostic and Therapeutic Implications of miRNA mediated Regulation of Prostate Cancer Progression”
Principal Investigator: Dr.PraveenkumarShetty
Co-Investigators:
Dr. Manaskumar Santra, NCCS, Pune
Dr. Rajashekar Mohan, Dept. of Surgery
Dr. Bhuvanesh Aradhya, Dept. of Urology
Rajiv Gandhi University of Health Sciences:
“Standardization of Ex-vivo cultured limbal stem cell transplantation – A collaborative study between ophthalmologist and cell scientist”
Principal Investigator: Dr. Praveenkumar Shetty,CRL/Dept. of Biochemistry
Co-Principal Investigator: Dr. Shankargouda Patil, Dept. of Ophthalmology
Co-Investigators: Dr.Vidya Patil, Dept. of Biochemistry
Dr. Mrudula Prabhu, Dept. of Ophthalmology
“To Evaluate the effect of different surface treatment on osteoblastic activity of Zirconia implant material- An in-vitro study”
Principal Investigator: Dr. Roseline Meshramkar, Dept. Of Prosthodontics, SDM College of Dental Sciences & Hospital, Dharwad
Co-PI: Dr. Praveenkumar Shetty
“Biocompatibility of Accelerated MTA plus on Human dental pulp stem cells”
Principal Investigator: Dr. Mahima Mohith, Dept. Of Conservative Dentistry, SDM College of Dental Sciences & Hospital, Dharwad
Co-PI: Dr. Praveenkumar Shetty
“Expression, Secretion and Excretory status of Insuline like growth factor 1 (IGF-1) and its correlation with cervical vertebral maturation stages in cleft lip and palate patients: A prospective study
Principal Investigator: Dr. Aditi V. Palankar, Dept. Of Oral and Maxillofacial Surgery, SDM College of Medical Sciences & Hospital, Dharwad
Co-PI: Dr. Praveenkumar Shetty
Induction of endometrial HOXA 10 gene expression and further improvement in the reproductive outcome by Vitamin D in Polycystic ovarian syndrome patients undergoing assisted reproduction techniques.
Principal Investigator: Dr. Shilpashree A.S, Dept. Of Biochemistry, SDM College of Medical Sciences & Hospital, Dharwad
Co-PI: Dr. Praveenkumar Shetty
Co-PI: Dr. Vinutha, Dept. Of Obstretics and Gynecology
Rheumatoid Arthritis Research Foundation (Private): “Identification of Proinflammatory Regulators and their Role in Pathogenesis of Rheumatoid Arthritis”
Principal Investigator: Dr.PraveenkumarShetty, CRL/Dept. of Biochemistry
Co-Principal Investigator: Dr.VikramHaridas, Dept. of Rheumatology
Academic funding from KBITS, Department of IT/BT, Government of Karnataka to start a Center for Biotechnology Skill Enhancement Programme (BiSEP)
Co-Ordinator: Dr. Praveenkumar Shetty
Backup Co-Ordinator: Dr. Vidya Patil, Professor and Head, Department of Biochemistry
RESEARCH PROJECTS UNDER PROGRESS
Identification and characterization of Novel Biomarkers and Therapeutic targets for Triple Negative Breast Cancer.(ICMR-Adhoc Project) Collaboration with department of Surgery Triple Negative Breast Cancer (TNBC) lacks the three widely used diagnostic marker (Her-2, ER and PR) and therefore these women are unable to benefit from the available therapies. According to practicing Oncologists, nearly 30-50% of new breast cancer cases, diagnosed in India are TNBC cases. TNBC tumors are highly destructive and the majority of deaths occur in the first 5 years. There is an urgent need to understand the mechanism of TNBC development, to identify novel biomarker and to develop targeted therapies for this group of women. Current research involving TNBC focuses on understanding the mechanisms of signaling cascade at the molecular level and therapeutic intervention targeting these over expressed biomarkers in cancer microenvironment.
miRNA mediated regulation of different tumor suppressors and oncogenes in Prostate Cancer progression. (DSt-SERB Project) Collaboration with Department of Surgery and urology Prostate cancer (PCa) is the second leading cause of cancer related death in men. It is the most prevalent tumor in men and despite increasing efforts at early detection, 10-20% of the cases present bone metastasis at diagnosis. Most men diagnosed with prostate cancer can survive the primary localized tumor, but because of the widespread metastasis, mortality rates remain extremely high. Androgen deprivation therapy is effective in initial stage PCa, but eventually, the disease progress to hormone resistant cancer, leading to death. However, a comprehensive understanding of the molecular signature of Metastatic Prostate Cancer is still lacking, and thus, further study of Metastatic PCa is an important step toward developing therapies for this lethal phenotype. Recent miRNA profiling studies associated specific miRNA expression with PCa progression. However, very few studies reported the role of specific miRNAs and respective targets in metastatic PCa development and progression. There is a great scope for intensive research into the identification of novel miRNAs, understanding their functional effects, so as to identify these novel biomarkers in carcinogenesis and their potential as therapeutic agents.
Standardisation of Ex-vivo Cultured Limbal Epithelial Stem Cell Transplantation - A Collaborative Study between Ophthalmologist and Cell Scientist(RGUHS-Advanced Research Project)Collaboratio with department of Ophthalmology Limbal stem cell deficiency (LSCD) constitutes a painful and potentially blinding condition with chronic epithelial defects, neovascularization, inflammation, ulceration, and corneal scarring, affecting about 10 million patients worldwide. On an average rate of two patients with LSCD are visiting our hospital every month. Corneal surface repair and visual rehabilitation in these patients can only be achieved by replenishing the depleted stem cell pool. Because of so many limitations autograft or allograft of LSCs are not advisible to these patients, which left with only ex-vivo expansion of LSCs and their transplantation. In the current study, we are focussing on standardization of ex-vivo expansion of LSCs and their characterization also we will try to improve safety, outcome, and search for alternate source to raise LSCs. So that we can take this technique to translational level and establish our center for LSC transplantation to serve poor patients of North Karnataka region.
“Identification of Proinflammatory Regulators and their Role in Pathogenesis of Rheumatoid Arthritis” Rheumatoid arthritis (RA) affects about 1% of global population, it is an inflammatory arthritis characterized by chronic synovitis. This chronic inflammatory synovitis is characterized by marked hyperplasia of the synovial lining cells and eventual degradation of cartilage and the subchondral bone. There is an extensive infiltration of granulocytes, macrophages, lymphocytes and fibroblasts in joints, where numerous inflammatory mediators, including interleukins, chemokines, growth factors, proteolytic enzymes and eicosanoids contribute to an ongoing process of inflammationThe exact pathogenesis of rheumatoid arthritis remains unclear although strong evidence suggests the involvement of cytokines in disease progression. There is no known cause of RA, but combination of genetic and environmental factors are the reason for the development of this disease continuum and would benefit from the development of new biomarkers and prognosticators.
Posttranscriptional regulation of BRCA1 in sporadic breast cancer.
Role of various biomarkers in Herceptin resistance and cancer recurrence.
Nanoparticle formulation and characterization of anti-diabetic and anti-cancer drugs.
Stem cell Research: in-vitro culture and characterization of chondrocytes extracted from cartilage biopsy (Orthopaedics) Skin graft derived melanocyte in-vitro culture and characterization. Role of Platelet Rich Plasma in the Management of Androgenic Alopaecia (Dermatology)
Ph.D Projects under progress:
In collaboration with Nitte University
Posttranscriptional regulation of BRCA1 in sporadic breast cancer. Mr. Anil Bargale, Dept. of Biochemistry
In Collaboration with BLDE University, Vijayapura “Plasminolytic Components and their Receptors in Pathogenesis of Preeclampsia., Mrs. Komal Ruikar, Dept. of Physiology
In collaboration with BLDE University, Vijayapura “Implications of Oral Contraceptive use to Phenotypic Expression Pattern of Receptors in Breast Cancer” Dr. Vittal Khode, Department of Physiology
In collaboration with BLDE University, Vijayapura “Comprehensive Immunophenotypic Expression Analysis of Phospholipid Binding Proteins in Renal Organogenesis and in Kidney Carcinoma” Dr. Roshini Sadashiv, Department of Anatomy
INSTITUTIONAL COMMITTEE LOOKING AFTER RESPONSIBLE CONDUCT OF RESEARCH
Institutional Ethical Committee
Institutional Bio Safety Committee
Institution Animal Protocol Committee
FACILITIES & TECHNIQUES
Animal and Human cell culture
Breast cancer sample repository
Immunohistochemistry facility with Image center
Western Blotting
DNA, RNA extraction and RTPCR
Recombinant Protein synthesis and purification
DNA and RNA binding studies
DNA damage studies
Various proliferation assays
Apoptosis assays
Gene Knock in & Knock out studies
Site directed Mutagenesis
Signalling /Elucidation of Molecular mechanisms of Oncogens and Tumor suppressors
In vivo animal studies (Cancer model)
Nanoparticle formulation & Characterization
RECENT PUBLICATIONS
Annexin A2/A1 interplay in the pathogenesis of Rheumatoid arthritis., VikramHaridas, Praveenkumar Shetty, Sharathkumar E, Anil Bargale, Jamboor K. Vishwanatha, Vidya Patil, Dinesh US., Internatonal Journal of Rheumatic Diseases. (In Press)
Comparative expression analysis of phospholipid binding protein AnnexinA1 in Nephrogenesis., Roshni Sadashiv, B.M. Bannur, Praveenkumar Shetty, U.S. Dinesh, Jamboor K. Vishwanatha., S.K.Deshpande, Anil Bargale, Sarathkumar . E, Komal Ruikar., Annals of Anatomy. (In Press)
HLA-B*27Allele Subtype Prevalence and Disease Association of Ankylosing spondylitis among South Indian Population., VikramHaridas., Praveenkumar Shetty., Nirmal Kumar M., Vasanthakumar KC., KiranHaridas., VitthalKhode., Anil Bargale., Indian Journal of Rheumatology., 2018; 13(1)., 38-43
Cdc20directs proteasome-mediated degradation of thetumor suppressor SMAR1 in higher grades of cancerthrough the anaphase promoting complex., Debasish Paul, SuvankarGhorai, US Dinesh, Praveenkumar Shetty, Samit Chattopadhyay and Manas Kumar Santra., Cell Death and Disease (2017) 8, doi:10.1038/cddis.2017.270
A microRNA/Ubiqutin Ligase feedback loop regulates Slug-mediated invasion in breast cancer., Rajesh Kumar Manne, Yashika Agrawal, Anil Bargale, Asha Patel, Debasish Paul, Neha Anilkumar Gupta, Srikanth Rapole, Vasudevan Seshadri, Deepa Subramanyam, Praveenkumar Shetty and Manas Kumar Santra., Neoplasia, 2017, Volume 19 Number xx pp. 483–495
Pyoderma gangrenosum in sjogrens syndrome and its successful treatment with topical application of Etanercept., VikramHaridas, Praveenkumar Shetty, Leonard Clinton Dsouza, Dinesh U. S, KiranHaridasand Anil Bargale, International Journal of Rheumatic Diseases. 2017; 20:657-659
Effects of salivary e-cadherin (CDH1-160) gene promoter polymorphism on the risk of development of oral cancer in Indian origin., Karkera, Namratha; Guttal, Kruthika ; Shetty, Praveenkumar; Bargale, Anil ; Kulkarni, Raghavendra; Kulkarni, Bhushan; Hiremath, Shivaprakash,, International Journal of Current Research., 2017., Vol. 9, Issue, 04, pp.49449-49454.
Glucose Induced activation of canonical wnt signaling pathway in hepatocellular carcinoma is regulated by DKK4.,SurbhiChouhan, Snahlata Singh, DiptiAthavale, Vimal Pandey, Jomon Joseph, Rajashekar Mohan, Praveenkumar Shetty and Manoj Kumar Bhat., Scientific Reports 6., DOI: 10.1038/srep27558
Annexin A2 and its Downstream IL-6 and HB-EGF as Secretory Biomarkers in the Differential Diagnosis of Her-2 Negative Breast Cancer., Praveenkumar Shetty*1, Vidya S Patil1, Rajashekar Mohan, Leonard Clinton Desouza1, Anil B1, Basavraj R. Patil, Dinesh U. S, VikramHaridas, Srirang Kulkarni1., Annals of Clinical Biochemistry 2016, 54(4)., 463-471
Cell Surface Interaction of Annexin A2 and Galectin-3 Modulates Epidermal Growth Factor Receptor Signaling in Her-2 Negative Breast Cancer Cells., PraveenkumarShetty, Anil B, Basavraj R. Patil, Rajashekar Mohan, Dinesh U. S, JamboorK.Vishwanatha, Pramod B. Gai, Vidya S Patil, Amsavardani TS., Mol Cell Biochemistry. 2016, 411 (1), 221-233
MIEN1, a novel interactor of Annexin A2, promotes tumor cell migration and invasion by stimulating plasmin generation Marilyne Kpetemey, Subhamoy Dasgupta, Smrithi Rajendiran, Susoban Das, Praveenkumar Shetty, Zygmunt Gryczynski, and Jamboor Vishwanatha, Molecular Cancer Research (2015) 14:156
Inhibition of triple-negative and Herceptin-resistant breast cancer cell proliferation and migration by Annexin A2 antibodies., P Chaudhary, S I Thamake, P Shetty and J K Vishwanatha., British Journal of Cancer., October 24, 2014; Doi: 10.1038/bjc.2014.542
Reciprocal regulation of AnxA2 and EGFR with Her-2 in Her-2 negative and Herceptin-resistant breast cancer, Praveenkumarshetty, Thamake Sanjay, Biswas S, Johansson S, Vishwanatha JK., PLOS ONE, 2012, Issue: 9, Volume-7., E44299.
Regulation of Urokinase expression at the posttranscriptional level in lung epithelial cells, Shwetha K. Shetty, Amarnath S. Marudamuthu, Daniel Abernathy, Rashmi S. Shetty, PraveenkumarShetty,Jian Fu, Steven Idell, Yashodhar P. Bhandary, HonglongJi, Ming-ChehLiu, and SreeramaShetty., Biochemistry, 2012, 51, 205-213.
Biotechnology Skill Enhancement Program –BiSEP An initiative of Department of IT, BT and S&T, Government of Karnataka
Supported by ABLE and The Department of Biotechnology, Government of India, New Delhi