Central Research Lab 

Central Research Laboratory has 1500 Sq. feet area, giving sufficient bench space for multiple investigators to work. Laboratory is well equipped to perform biomedical research in various fields like Molecular and Cellular biology, Immunology, Biochemistry and Nanoparticle formulation and Characterization. The scope of the laboratory is to cater the research needs of graduate and postgraduate students and also interested faculty from all the departments of the Institution.
CENTRAL RESEARCH LABORATORY COMMITTEE
  • Chairman: Dr. Niranjankumar, Medical Director
  • Co-Ordinator: Dr. J. V. Chowti, Principal
  • Research Co-Ordinator: Dr. Praveenkumar Shetty   Short CV
  • Members:
    • Dr. Satyashankar, Medical Superintendent
    • HOD, Pathology
    • HOD, Biochemistry
    • HOD, Pharmacology
    • HOD, Microbiology
 NATIONAL & INTERNATIONAL COLLABORATIONS AT INVESTIGATOR LEVEL
  1. UNT Health Science Center at Fort-Worth, Texas, USA
  2. National Centre for Cell Sciences, Pune.
  3. K. S. Hegde Institute of Medical Sciences, Nitte University, Mangalore.
  4. Karnataka Cancer Therapy & Research Institute, Hubli
  5. Karnataka University, Dharwad.
CURRENT FUNDING
ICMR Ad-hoc Research Project:
  1. “Identification and characterization of Novel Biomarkers and    Therapeutic targets for Triple Negative Breast Cancer”
    Principal Investigator: Dr.Praveenkumar Shetty
    Co-Investigators:
    Dr. B. R. Patil, Karnataka cancer Therapy and Research institute, Hubli
    Dr. Col. U. S. Dinesh, Dept. of Pathology, SDMCMSH
    Dr.Pramod B. Gai, DNA Diagnostic laboratotry, Dharwad
  2. Recently sanctioned ICMR Ad-hoc Project: “Diagnostic and Therapeutic Implications of miRNA mediated Regulation of Prostate Cancer Progression”
    Principal Investigator: Dr.Praveenkumar Shetty
    Co-Investigators:
    Dr.Rajashekar Mohan, Dept. of Surgery
    Dr.SangamnathBentur, Dept. of Urology
    Dr.Shubada.C, Dept. of Microbiology
  3. Rajiv Gandhi University of Health Sciences: 2015-2017., “Standardization of Ex-vivo cultured limbal stem cell transplantation – A collaborative study between ophthalmologist and cell scientist”
    Principal Investigator: Dr.Praveenkumar Shetty,CRL/Dept. of Biochemistry
    Co-Principal Investigator: Dr. ShankargoudaPatil, Dept. of Ophthalmology
    Co-Investigators:  Dr. VidyaPatil, Dept. of Biochemistry
    Dr. MrudulaPrabhu,Dept. of Ophthalmology
  4. Rheumatoid Arthritis Research foundation (private): 2015-2018., “Identification of Proinflammatory Regulators and their Role in Pathogenesis of Rheumatoid Arthritis”
    Principal Investigator: Dr. Praveenkumar Shetty, CRL/Dept. of Biochemistry
    Co-Principal Investigator: Dr. VikramHaridas, Dept. of Rheumatology
  5. Institutional funding & Seed funding from Karnataka Cancer Therapy & Research Institute Hubli
     
RESEARCH PROJECTS UNDER PROGRESS
 
  1. Identification and characterization of Novel Biomarkers and Therapeutic targets for Triple Negative Breast Cancer.(ICMR-Adhoc Project) Collaboration with department of Surgery Triple Negative Breast Cancer (TNBC) lacks the three widely used diagnostic marker (Her-2, ER and PR) and therefore these women are unable to benefit from the available therapies. According to practicing Oncologists, nearly 30-50% of new breast cancer cases, diagnosed in India are TNBC cases. TNBC tumors are highly destructive and the majority of deaths occur in the first 5 years. There is an urgent need to understand the mechanism of TNBC development, to identify novel biomarker and to develop targeted therapies for this group of women. Current research involving TNBC focuses on understanding the mechanisms of signaling cascade at the molecular level and therapeutic intervention targeting these over expressed biomarkers in cancer microenvironment.
  2. miRNA mediated regulation of different tumor suppressors and oncogenes in Prostate Cancer progression. (ICMR-Adhoc Project) Collaboration with Department of Surgery and urology Prostate cancer (PCa) is the second leading cause of cancer related death in men. It is the most prevalent tumor in men and despite increasing efforts at early detection, 10-20% of the cases present bone metastasis at diagnosis. Most men diagnosed with prostate cancer can survive the primary localized tumor, but because of the widespread metastasis, mortality rates remain extremely high. Androgen deprivation therapy is effective in initial stage PCa, but eventually, the disease progress to hormone resistant cancer, leading to death. However, a comprehensive understanding of the molecular signature of Metastatic Prostate Cancer is still lacking, and thus, further study of Metastatic PCa is an important step toward developing therapies for this lethal phenotype. Recent miRNA profiling studies associated specific miRNA expression with PCa progression. However, very few studies reported the role of specific miRNAs and respective targets in metastatic PCa development and progression. There is a great scope for intensive research into the identification of novel miRNAs, understanding their functional effects, so as to identify these novel biomarkers in carcinogenesis and their potential as therapeutic agents.
  3. Standardisation of Ex-vivo Cultured Limbal Epithelial Stem Cell Transplantation - A Collaborative Study between Ophthalmologist and Cell Scientist(RGUHS-Advanced Research Project)Collaboratio with department of Ophthalmology Limbal stem cell deficiency (LSCD) constitutes a painful and potentially blinding condition with chronic epithelial defects, neovascularization, inflammation, ulceration, and corneal scarring, affecting about 10 million patients worldwide. On an average rate of two patients with LSCD are visiting our hospital every month. Corneal surface repair and visual rehabilitation in these patients can only be achieved by replenishing the depleted stem cell pool. Because of so many limitations autograft or allograft of LSCs are not advisible to these patients, which left with only ex-vivo expansion of LSCs and their transplantation. In the current study, we are focussing on standardization of ex-vivo expansion of LSCs and their characterization also we will try to improve safety, outcome, and search for alternate source to raise LSCs. So that we can take this technique to translational level and establish our center for LSC transplantation to serve poor patients of North Karnataka region.
  4. Posttranscriptional regulation of BRCA1 in sporadic breast cancer.
  5. Role of various biomarkers in Herceptin resistance and cancer recurrence.
  6. Nanoparticle formulation and characterization of anti-diabetic and anti-cancer drugs.
  7. Glioma classification according to molecular biomarker status and their therapeutic implications. Collaboration with Department of Neuro surgery
  8. Study to validate the protective function of novel peptide against acinar cell necrosis in experimental pancreatitis.
  9. Stem cell Research: in-vitro culture and characterization of chondrocytes extracted from cartilage biopsy (Orthopaedics) Skin graft derived melanocyte in-vitro culture and characterization.Role of Platelet Rich Plasma in the Management of Androgenic Alopaecia (Dermatology)
 
Completed ICMR-STS Project: “Animal Lectin Galectin-3 as a Potential Biomarker in Oral Squamous Cell Carcinoma” Student: Miss. V.P. Sreelakshmi (2014-2015)S
Ph.D work (Current- Nitte University): Title: Posttranscriptional regulation of BRCA1 in sporadic breast cancer., Mr. Anil Bargale, Dept. Of Biochemistry
MDS Dissertation work (Completed-RGUHS):
 
  1. Title: Estimation and Correlation of serum and Salivary Glucose, Ig A level and Salivary Candidial Carriage in Diabetic and in Non-Diabetic Patients, Dr.ShrutiHegde, Dept. Of Oral Medicine and Radiology
  2. Title: Assessment of Serum C-Reactive protein Levels as a biomarker in patients with Oral Potentially Malignant Lesions-A Prospective study, Dr.FazeelaFarzana A, Dept. Of Oral Medicine and Radiology
 
MDS Dissertation work (Current-RGUHS):
  1. Biological evaluation of root repair materials by cytotoxicity study using periodontal ligament fibroblasts
  2. Effects of E-Cadherin (CDH1-160) Gene promoter Polymorphism on the Risk of Development of Oral Cancer in North-Karnataka Population
INSTITUTIONAL COMMITTEE LOOKING AFTER RESPONSIBLE CONDUCT OF RESEARCH
  • Institutional Ethical Committee
  • Institutional Bio safety Committee
  • Institution animal protocol committee
FACILITIES & TECHNIQUES
  • Animal and Human cell culture
  • Breast cancer sample repository
  • Immunohistochemistry facility with Image center
  • Western Blotting
  • DNA, RNA extraction and RTPCR
  • Recombinant Protein synthesis and purification
  • DNA and RNA binding studies
  • DNA damage studies
  • Various proliferation assays
  • Apoptosis assays
  • Gene Knock in & Knock out studies
  • Site directed Mutagenesis
  • Signalling /Elucidation of Molecular mechanisms of Oncogens and Tumor suppressors
  • In vivo animal studies (Cancer model)
  • Nanoparticle formulation & Characterization
RECENT PUBLICATIONS
  1. Cell Surface Interaction of Annexin A2 and Galectin-3 Modulates Epidermal Growth Factor Receptor Signaling in Her-2 Negative Breast Cancer Cells. Praveenkumarshetty, Anil B, Basavraj R. Patil, Rajashekar Mohan, Dinesh U. S, JamboorK.Vishwanatha, Pramod B. Gai, Vidya S Patil, Amsavardani T S.DOI 10.1007/s11010-015-2584-y(2015),Molecular and Cellular Biochemistry
  2. MIEN1, a novel interactor of Annexin A2, promotes tumor cell migration and invasion by stimulating plasmin generation MarilyneKpetemey, SubhamoyDasgupta, SmrithiRajendiran, Susoban Das, Praveenkumar Shetty,ZygmuntGryczynski, and JamboorVishwanatha, Molecular Cancer Research (2015) 14:156
  3. Inhibition of triple-negative and Herceptin-resistant breast cancer cell proliferation and migration by Annexin A2 antibodies., P Chaudhary, S I Thamake, P Shetty and J K Vishwanatha., British Journal of Cancer., October 24, 2014; Doi: 10.1038/bjc.2014.542
  4. Reciprocal regulation of AnxA2 and EGFR with Her-2 in Her-2 negative and Herceptin-resistant breast cancer, PraveenkumarshettyThamake Sanjay, Biswas S, Johansson S, Vishwanatha JK., PLOS ONE, 2012, Issue: 9, Volume-7., E44299.
  5. Regulation of Urokinase expression at the posttranscriptional level in lung epithelial cells, Shwetha K. Shetty, Amarnath S. Marudamuthu, Daniel Abernathy, Rashmi S. Shetty, Praveenkumar Shetty,Jian Fu, Steven Idell, Yashodhar P. Bhandary, Honglong Ji, Ming-ChehLiu, and Sreerama Shetty., Biochemistry, 2012, 51, 205-213.
 

 

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