Biochemistry

About the Department

Knowledge of Biochemistry is essential to all the branches of medicine. Biochemical approaches have illuminated many aspects of health and disease. Health depends on a harmonious balance of biochemical reactions occurring in the body and disease reflects abnormalities in bio molecules, biochemical process and reactions. In our medical college, biochemistry department caters to the needs of students by involving in teaching activities, while in the hospital the clinical biochemistry department performs the laboratory investigations to serve the needs of patients for correct diagnosis. The department of Biochemistry possesses excellent infrastructure. Dedicated full time teaching faculty is a mixture of experienced and young enthusiastic teachers. Besides teaching and diagnostic work, the faculty is actively involved in research projects in the fields of current clinical interests.

 

Head of the Department

Department of Biochemistry is headed by Dr. Vidya S Patil. She has done her undergraduation from JJMMC, Davangere and M.D Biochemistry from J N Medical College Belgaum (RGUHS). She has 10 years of teaching experience. She has many research papers for her credit published in national and international journals. She is a recognized Post graduate guide from RGUHS and currently one of the BOS members for Preclinical P.G curriculum. She is a life member of national and state associations in the field of Biochemistry. She has also worked as joint secretary for Karnataka chapter of Association of Medical Biochemists of India during 2013-14.

 

Diagnostic Facilities

Clinical biochemistry lab is situated in the Hospital building with 24X7 stat lab facilities and has two sections- routine clinical chemistry and endocrinology. It was started in September 2003. At present this laboratory processes 450 samples with 2000 investigations every day. The Lab is committed to providing a high quality and efficient service to its users.

Total quality performance of the Lab is monitored by participating in the External Quality Accreditation programme like Biorad EQAS run by Biorad, USA and EQAS programme run by CMC, Vellore. Internal Quality assurance is done by performing daily controls from Biorad, USA.

The Lab is equipped with the following instruments

  1. Fully automated chemistry analyzers – Siemens Dimension RXL Max
  2. Fully automated chemistry analyzers – Siemens Dimension RXL Max (standby)
  3. Fully automated hormone  analyzers – Siemens ADVIA CENTAUR CP immunoassay analyser (Chemiluminescence method)
  4. Fully automated HbA1c analyzer (HPLC method) – Biorad D10
  5. ABG analyser- Roche COBAS b121
  6. Electrolyte analyser- Roche AVL9180
  7. Semi automated Chemistry analyser- Biosystems BTS-350

Special investigations like Serum protein Electrophoresis, Hemoglobin Electrophoresis (semi-automated Helena electrophoresis equipment), screening for IEM, stone analysis is also performed.

The Lab has also installed the LIS (Laboratory Information System) through which the specimens are bar-coded, tested and the results are automatically sent online from Lab to OPDs , wards and to end users. This speeds up the test reporting and also minimizes transcriptional errors.

 

Teaching Program

Department is well equipped with demonstration room and practical hall. 

  1. Undergraduate: Classes, seminars, tutorials, tests are conducted regularly for the benefit of students. Proper care of pupils is taken through the teacher guide programmes.
  2. Post-graduate: 4 PG seats have been allotted by MCI from the year 2014. We have adequate infrastructure to train the PGs in various techniques like:
    • Electrophoresis
    • Chromatography (HPLC from schimatzu)
    • Blotting technique
    • Cell and tissue culture

We Train PGs in the laboratory skills by posting them in clinical laboratory, clinical postings in various department have been scheduled for refinement of the knowledge of the postgraduate.

 

Workshops and CMEs

Department puts its constant effort to update the faculty by conducting regular CME/ Workshop on current topics.

  1. Department of Biochemistry conducted CME/Workshop on Electrophoresis -A versatile analytical tool” on Saturday 17th September 2011.
  2. Conducted CME/workshop on “Proteomics-expression analysis” Techniques and Methodology in biomedical research, on 8th October 2014 

 

Publications
  1. Praveenkumar Shetty, Anil Bargale, Basavaraj R Patil, Rajshekhar Mohan, U.S Dinesh, Jamboor K Vishwanatha, Pramod B Gai, Vidya S patil, TS Amsavardani. Cell surface interaction of annexin A2 and galectin 3 modulates epidermal growth factor receptor signaling in Her-2 negative breast cancer cells. MolCellBiochem .2016;411(1-2):221-33.
  2. Vijayetha P. Patil, , Anuradha B. Patil, Vidya S. Patil, Deepti G. Ingleshwar. Paraoxonase Activity and Lipid Profile in Paediatric Nephrotic Syndrome: A Cross-sectional Study. Journal of Clinical and Diagnostic Research. 2016;10(3): BC17-BC20.
  3. Vidya.S.Patil, Vijayetha P. Patil, NeerajaGokhale, AnirudhAcharya, Praveenchandrakangokar. Chronic Periodontitis in Type 2 Diabetes Mellitus: Oxidative Stress as a Common Factor in Periodontal Tissue Injury. Journal of Clinical and Diagnostic Research. 2016;10(4): BC12-BC16.
  4. Surbhi Chouhan, Snahlata Singh, Dipti Athavale, Pranay Ramteke, Vimal Pandey, Jomon Joseph, Rajashekar Mohan, Praveen Kumar Shetty, Manoj Kumar Bhat. Glucose induced activation of canonical Wnt signaling pathway in hepatocellular carcinoma is regulated by DKK4. Scientific Reports/6:27558/DOI: 10.1038/srep27558
  5. Shreerang Pralhad Kulkarni, Rakesh Mudaraddi, Dhiraj J Trivedi, Pramod S Kamble. Association of Thyroid dysfunction in patients with type-2 diabetes mellitus. International Journal of Clinical Biochemistry and Research. 2016; 3(2): 172-176.
  6. Manohara A Bhat, KVV Prasad, Dhiraj Trivedi, BR Rajiv, Hemant Battur. Pyruvic acid levels in serum and plasma: A new course for oral cancer screening ? Journal of Oral and Maxillofacial Pathology. 2016; 20 (1): 102-105.
  7. Dhiraj J Trivedi, Bandi Joshi Raj, Vijay Bidkar, Raghavendra Rao. Methemoglobinemia: Living with Dormant Devil. Ind J ClinBiochem. DOI 10.1007/s 12291-016-0586-5.
  8. Rama Jailkhani, Shivashankara Arnadi Ramachandrayya, Vidya Shankargouda Patil, Sameena. A hospital-based study of prevalence of thyroid dysfunction in Srinagar, Jammu and Kashmir state of India. International Journal of Medical Sciences & Public Health.2015;4(2):151-154.
  9. Praveenkumar Shetty, Anil B, Basavraj R Patil, Rajashekar Mohan, Dinesh U S, Jamboor K Vishwanatha,  Pramod B Gai, Vidya S Patil, Amsavardani TS. Cell Surface Interaction of Annexin A2 and Galectin-3 Modulates Epidermal Growth Factor Receptor Signaling in Her-2 Negative Breast Cancer Cells. Mol Cell Biochemistry. 2016 Jan;411(1-2):221-33.
  10. Rakesh Mudaraddi, Shrirang Prahlad Kulkarni, Dhiraj Jayantilal Trivedi, Vidya Shankargouda Patil, PramodSudamKamble. Association of Serum Electrolytes and Urea Levels with Cardiac Markers in Acute Myocardial Infarction. International Journal of Clinical Biochemistry Research. 2015; 2(4): 234-235.
  11. Dhiraj J Trivedi, Vikram Haridas. Five Cases of Alkaptonuria Among Two Generations of Single Family in Dharwad, Karnataka (India). Ind J Clin Biochem;  2015, 30(4):479-484.
  12. Kaveri Hallikeri, Swetha Acharya, Anila Koneru, Dhiraj J Trivedi. Evaluation of microvessel density in central and peripheral giant cell granulomas. J AdvanClin& Res Insights. 2015; 2(1):20-25.
  13. Tanvee S Wadikar, Swati B Setty, Kishore G Bhat, Dhiraj J Trivedi, Srinath L Thakur. Antibacterial activity of aqueous extract of benincasahispida fruit  against periodontal pathogens. Int J of sci study 2015; 3(1):145-149.
  14. Akshata Raghavendra Okade, Kaveri Satish Hallikeri, Dhiraj Jayantilal Trivedi. Salivary estimation of copper, iron, zinc and manganese in oral submucous fibrosis patients: A case- control study. Clinical cancer investigation journal. 2015; 4(3): 302-306.
  15. Dhiraj J Trivedi, Prashanth Naik. Alkaptonuria: A Case of Familial Inheritance from Hangarki Village in Dharwad District of Karnataka: Ind. J. Clinical Biochemistry. 2016,31(3):353-356
  16. Anithraj, Manohara Bhat, Kakarla Prasad, Dhiraj Trivedi, Swathi Acharya. Esimation of Pyruvic acid in serum and saliva among healthy and potentially malignant disorder subject- a stepping stone for cancer screening ?.  J. of Clinical & Exp. Dent. 2015, 7(4): 462-5.
  17. Shindhe Vijayalaxmi, Trivedi Dhiraj, Narav Asha. Garbhasanskar: Knowledge, Attitude and Practice among Antenatal Mothers of Dharwad, Karnataka. Int.J. of Advances in Nursing Management. 2015, 3(4):319-324.
  18. Marilyne Kpetemey, Subhamoy Dasgupta, Smrithi Rajendiran, Susobhan Das, Lee Gibbs, Praveenkumar Shetty, Zygmunt Gryczynski, Jamboor Vishwanatha. MIEN1, a novel interactor of Annexin A2, promotes tumor cell migration by enhancing AnxA2 cell surface expression. Molecular Cancer. 2015; 14:156.
  19. Vani Axita Chandrakant, Hemanth Kumar R G – a pattern of acute poisoning in Dharwad- India. Medico legal update. 2015; 15(1): 9-12.
  20. Vani Axita Chandrakant, Santosh Kumar, Hemanth Kumar R G-Homocysteine levels in diabetic patients with and without cardiovascular disease: A Cross sectional analytical study. International Journal of  Pharma and Biosciences. 2015; 6 (2): 1361-1365. 
  21. Pranali P Karne, Shankar M. Pawar, Anil B Bargale. Study of lipid profile in Psoriasis patients. International Journal of Clinical Biochemistry and Research. 2015; 2(1):1-4.
  22. ManasaHosamane, Anirudh B. Acharya, ChhaviVij, DhirajTrivedi. Evaluation of holy basil mouthwash as an adjunctive plaque control agent in a four day plaque regrowth model. J ClinExp Dent. 2014; 6(5): e491-6.
  23. Dhiraj J Trivedi, Chandru MC, Akshata Shinde, Chhaya D Trivedi. Alcohol impact on membrane glycoprotein: a lectin study. Int. J. Int sci. Inn. Tech. Sec. B. 2014; 3(5): 12-15.
  24. Deepti G ingleshwar, Sukanya Shetty, Ashalatha V Rao. Age related difference in the lipid profile in normal healthy woman. Niite university Journal of Health Science. 2014; 4(2): 94-97.
  25. Hemanth Kumar R G, Vani Axita Chandrakant, Pratibha. Blunt chest trauma induced acute myocardial infarction- Medico legal update. 2014; 14(2): 143-145.
  26. Shilpasree AS, Kirankumar. Role of ischemia modified albumin in the diagnosis of acute coronary syndrome. IJPBS. 2014: 3(6):168-173.
  27. Neeraja H Gokhale, Anirudh B Acharya, Vidya S Patil, Dhiraj J Trivedi, Swathi Shetty, Srinath L Thakur. Resistin levels in GCF in chronic Periodontitis Patients with Type 2 Diabetes Mellitus. J Periodontol. 2014; 85(4):610-7
  28. Prakash D Zende, Mangesh P. Bankar, Abdul Rahman Momin,  Pramod Kamble. Study of Cholesterol Ester Transfer Protein 1405v genotype and its association  with lipid fractions in myocardial infarction patients: A case control study. Journal of Clinical and Diagnostic Research 2014 jun; 8 (6): cc01-cc04
  29. Dhiraj Trivedi, Aparna Sagare. Assessment of iron overload in homozygous ant heterozygous beta thalassemic children below 5 years of age. JKIMSU 2014; 3(2)
  30. P. Chaudhary, S I Thamake, P. Shetty , J K Vishwanatha. Inhibition of triple negative and Herceptin resistant breast cancer cell proliferation and migration by annexin A2 antibodies. British Journal of cancer 2014;111(12):232841.doi:10.1038/bjc2014542
  31. Gokhale. NH, Acharya .AB, Patil VS, Trivedi DJ, Thakur SL. A short-term evaluation of the relationship between plasma ascorbic acid levels and periondontal disease in systemically healthy and type 2 diabetes mellitus subjects. J  Diet Suppl. 2013; 10(2): 93-104
  32. Deepti G ingleshwar, Sukanya Shetty, Ashalatha V Rao. Age related difference in the lipid profile in normal healthy woman. Niite university Journal of Health Science 2014;4(2):94-97
  33. Dhiraj J Trivedi, Divya M, Priyanka, Manali Bhat, Vidya BU. Cord serum bilirubin and albumin in neonatal hyperbilirubinemia. Int J of integrative sciences, Innovation and Technology IJIIT. 2013,2(2):39-42.
  34. Kiran Patil1, Dhiraj J Trivedi, K Sankara Narayan. Postmortem Blood Glucose (BlGlu) Level: An Attempt to Estimate Time since Death IJFPR. Vol. 03, No: 02 (July-Dec), 2013
  35. Dhiraj J Trivedi. Oxidant and antioxidant imbalance as root cause in preeclampsia. Trans world medical Journal. 1(2):33-36
  36. Chandramati J and Dhiraj J Trivedi. Health related problems in Menopausal women of north Karnataka  ,IJIIT. 2013;2(4).page no44-46 ISSN 2278
  37. Pramod Kamble, Bankar M P, Prakash Zende, Dhiraj Trivedi, Abdul Rahman Momin. Impact of homocysteine, B-vitamin in patients with Arteriosclerosis. Research Journal of pharmaceutical, biological and chemical science 2013; 4(2):344-348
  38. Aparna A Sagare, Dr.Jayashree V Ganu, Dr.Dhiraj  JTrivedi, Pramod S Kamble, Anil Bargale. Effect of traditional Biochemical markers on endothelial dysfunction in preeclampsia. International Journal of Pharma and Biosciences. 2012; 3(3):B16-21.
  39. DhirajTrivedi, Salivary proteome in periodontal diagnosis-International Journal of Pharma and Biosciences  2012 ;3(2):0975-6299.
  40. DhirajTrivedi, Salivary sialic acid as marker of oral cancer. International Journal of integrative sciences, innovation and Technology. 2012;1(1):48-50 
  41. Vidya S Patil, Rama Jailkhani, DhirajTrivedi, Shreerang P Kulkarni, Aparna A Sagare, RakeshMudaraddi, Anil Bargale. Screening for aminoacidurias and organic acidurias in patients with metabolic or neurological manifestations. Biomedical Research 2012; 23(2):253-258.
  42. ShettyPraveenkumar, Thamake Sanjay, Biswas S, Johansson S, Vishwanatha JK Reciprocal regulation of AnxA2 and EGFR with Her-2 in Her-2 negative and Herceptin-resistant breast cancer.(PLOS ONE, August 2012, accepted in press)
  43. Shwetha K. Shetty, Amarnath S. Marudamuthu, Daniel Abernathy,  Rashmi S. Shetty,  PraveenkumarShetty,  Jian Fu,  Steven Idell, Yashodhar P. Bhandary, HonglongJi, Ming-ChehLiu,andSreeramaShetty. Regulation of Urokinase expression at the posttranscriptional level in lung epithelial cells, Biochemistry. 2012, 51, 205-213
  44. Ramesh, RakeshMudaraddi, RavindraMaradi. Serum ceruloplasmin-albumin ratio as a biochemical marker to assist the diagnosis, treatment and prognosis of pulmonary tuberculosis patients. Research Journal of Pharmaceutical, Biological and Chemical Sciences 2012;3(2):494-499
  45. Vijay R Pandhare, Pramod.S.Kamble, Prakash D Zende. Impact of antioxidant activity on enzymes of seminal plasma in association with mental stress subjects. International Journal of Pharma and Biosciences. 2012;3(3):1025-1031
  46. Aparna A Sagare, Dhiraj.J.Trivedi, Vidya.S Patil, Shreerang P. Kulkarni. Protein Carbonyl & Microalbuminuria in type2 Diabetes Mellitus. Ind J B & A Med Res, 2012;  5(2): 399-404
  47. S. Gowda, P.B. Desai , V.S. Kagwad , S.J. Shetty ,M.B. Ilakal. Ijpsr. Variants Of Oral Glucose Tolerance Test (Ogtt) Curve In Gestational Diabetes Mellitus (Gdm) 2012; Vol. 3(10): 3966-3970
  48. Dr. Tejasvini gouripur , Dr. P. B. Desai, Dr. Axita vani , Dr. Kranti gouripur, Dr. Vijayeta patil. Comparison Of Lipid Peroxidation Product And Enzymatic Antioxidants In Newly Diagnosed Pulmonary Tuberculosis Patients With And Without Human Deficiency Virus Infection. Int j pharm bio sci 2012 july; 3(3): (b) 391 – 397
  49. DhirajTrivedi, ChhayaTrivedi, PramodKamble, M C Chandru. Lectin based Hemagglutination  assay : A screening test for thyroid disorders. International Journal of Pharma and Biosciences.2011; 2 (4):515-518.
  50. Dr Dhiraj Trivedi, Chhaya Trivedi, Anil B Bargale. Altered expression of pedilanthus tithymaloidus lectin binding glycoprotein on erythrocytes in thyroid disorders, a preliminary report. International Journal of Pharma and Biosciences. 2011;2(3):411-415.
  51. Indumati V, Vidya S Patil, Krishnaswamy D, Satishkumar D, Vijay V, Mahesh S, Rajeshwari V. Non –HDL cholesterol and LDL-C/HDL-C Ratio In type II diabetic patients: International Journal of Pharma and Biosciences 2011;2(2):B-71-77.
  52. Vidya S Patil, P B Desai , M V Kodliwadmath, Indumati V. Utility of gamma glutamyltrasferase and AST/ALT (De Ritis) ratio in alcoholic liver diseases. International journal of Medical Sciences and Technology  2011,Vol 4(1);1-5.
  53. Rakesh Mudaraddi, Ramesh, Sameer, Amareshwara M, Ravindra Maradi, Anil B and Beena V. Shetty   Evaluation of oxidative stress and antioxidant status in patients with epilepsy International Journal of Pharma and Biosciences. 2011; 2 (3):303-308
  54. Ramesh, Sudha k, Amareshwara M, Smeer and Rakesh M. Study of protein oxidation and antioxidants status in pulmonary tuberculosis  patients. International Journal of Pharma and Biosciences, 2011; 2(3):104-109.
  55. Vivek R Joshi, Ravindra Maradi, Ayaz K. Mallick, Rakesh Mudaraddi, Ramesh Hadapad, Vinay Khanna. Relationship between Glycated Hemoglobin Levels and the Iodine uptake in Patients with Diabetes Mellitus.Research Journal of Pharmaceutical, Biological and Chemical Sciences 2011;2(3):107-111
  56. Amareshwara M, Gayathri M Rao, Rakesh M, Ramesh, Sameer, Shreekantha. Study of protein oxidation products and antioxidants in primary brain tumour patients. International Journal of Pharma and Biosciences 2011;2(1):B-256-261
  57. Anil Bargale, Jayashree V. Ganu, Dhiraj J. Trivedi, Nitin Nagane, Rakesh Mudaraddi, Aparna Sagare. Serum HS-CRP and uric acid as indicator of severity in preeclampsia . International Journal of Pharma and Biosciences. 2011; 2( 3): 340-345.
  58. Anil B Bargale, Jayashree V Ganu, Dhiraj J Trivedi, Pramod S Kamble, Rakesh Mudaraddi. Serum superoxide dismutase and paraoxonase-1 activity in preeclampsia patients. International Journal of Pharma and Biosciences 2011;2(4):705-709.
  59. Pramod Kamble, Dhiraj J Trivedi, Anil Bargale, Vijay Pandhare,Prakash Zende,Pradnya Padalkar. Role of xanthine oxidase, lipid peroxidation and uric acid in patients with acute myocardial infarction. International Journal of Pharma and Biosciences 2011; 2 (4):395-399.
  60. Pramod Kamble, Vinod Bhagwat, Dhiraj J Trivedi, Anil Bargale. Comparative study of free radical activity in plasmodium falciparum and plasmodium vivax malaria patients - International Journal of Pharma and Biosciences 2011; 2 (4): 99-102.
  61. Pramod K. Feeding Practices and neonatal Hperbilirubinemia. The Antiseptic 2011;108 (8):379-381
  62. Shivaraj Gowda, Prakash B Desai, Sindhu J Shetty, Vijayetha S Kagwad, Mahantesh B Ilakalijph. Malarial Hepatitis And Renal Failure: A Study Of Two Cases 2011; 8(1)
  63. Jailkhani R , Patil V S, Kulkarni S P, Pervatikar S, Jayashankara B B.an interesting case of compound heterozygous sickle cell-β+ thalassemia presenting with acute chest syndrome. Journal of Clinical and Diagnostic Research 2010; 4: 2291-2296.
  64. Jailkhani R, Patil V S., Laxman H B, Shivashankara A R, Kulkarni S P, Ravindra M S. selective screening for inborn errors of metabolism-single center experience from Karnataka. Journal of Clinical and Diagnostic Research 2008 Aug; (2): 912-918
  65. Shreerang P Kulkarni, C R Mallikarjuna, D S Jayaprakash Murthy. Cerebrospinal fluid free sialic acid and aspartate transaminase levels in meningitis.  Indian Journal of Clinical Biochemistry  2006; 21(1): 185-188

 

Dissertation/ Research
  1. Dr. Praveenkumar Shetty- “Identification and Characterization of Novel biomarker & Therapeutic target for Triple Negative Breast Cancer” (Seed Research grant from Karnataka cancer therapy and Research centre).
  2. Dr. P.K. Shetty-  “miRNA mediated regulation of Oncogenes and Tumor Suppressors in Prostate cancer progression”.
  3. Significance of oxidants –antioxidants status in oral squamous cell  carcinoma patients- A case control study by Dr. Gokul S, MDS student ,Dept. of oral pathology, SDM Dental college,  Guided by Dr. Vidya S Patil. (Completed in 2009)
  4. Post-graduate dissertation: Study of urinary glycosaminoglycans among essential hypertensive patients by Dr Sanjeevini N. B. Dept of Biochemistry, SDM college of Medical sciences and Hospital Dharwad, Guide is Dr. Dhiraj Trivedi.

 

Faculty:
1
Dr. Vidya S. Patil
Professor & HOD
2
Dr. Dhiraj J. Trivedi
Professor (Phd)
3
Dr. Shreeranga P. Kulkarni
Professor
4
Dr. Praveen Kumar Shetty B.          
Professor (Phd)
5
Dr. Rakesh T. Mudaraddi
Associate Professor
6      
Dr. Vani Axita Chandrakant
Associate Professor
7
Dr. Vijayetha Preetam Patil
Associate Professor
8
Dr. Deepti G Ingleshwar
Assistant Professor
9
Dr. Shilpasree A S
Assistant Professor
10
Mr. Kamble Pramod Sudam
Tutor  (Msc)
11
Mr. Bargale Anil B.
Tutor  (Msc)
12
Dr. Sanjeevini N B
Tutor